bsmbi v2 digested plasmid backbone (New England Biolabs)
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bsmbi v2 digested plasmid backbone
Bsmbi V2 Digested Plasmid Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsmbi+v2+digested+plasmid+backbone/BsmBI-v2/bio_rxiv__2025__11__14__688347-230-21-16
Average 99 stars, based on 1624 article reviews
Bsmbi V2 Digested Plasmid Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsmbi+v2+digested+plasmid+backbone/BsmBI-v2/bio_rxiv__2025__11__14__688347-230-21-16
Average 99 stars, based on 1624 article reviews
bsmbi v2 digested plasmid backbone - by Bioz Stars,
2026-09
99/100 stars
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Plasmid Preparation:Article Title: Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis Article Snippet: Briefly, the CRISPRi plasmid backbone (see ) was digested with BsmBI-v2 (NEB #R0739L) and gel purified. sgRNAs were designed to target the non-template strand of the target gene ORF. .. For each individual sgRNA, two complementary oligonucleotides with appropriate sticky end overhangs were annealed and ligated (T4 ligase NEB # M0202M) into the Article Title: The phenotypic landscape of the mycobacterial cell Article Snippet: Briefly, the CRISPRi plasmid backbone was digested with BsmBI-v2 (NEB #R0739L). .. For each individual sgRNA, two complementary oligonucleotides with appropriate overhangs were annealed and ligated (T4 ligase NEB # M0202M) into the Article Title: Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria. Article Snippet: For cloning, CRISPRi plasmids were digested with BsmBI-v2 (NEB #R0739L) and further gel purified (Macherey-Nagel, Hoerdt, France; #740609.50). .. For each individual single-guide ribonucleic acid (sgRNA) targeting sequence, two complementary annealed oligonucleotides with appropriate sticky end overhangs were ligated (NEB; #M0202M) into the desired Article Title: Fluorescence‐based CRISPR interference system for controlled genetic repression and live single‐cell imaging in mycobacteria Article Snippet: For cloning, CRISPRi plasmids were digested with BsmBI‐v2 (NEB #R0739L) and further gel purified (Macherey‐Nagel, Hoerdt, France; #740609.50). .. For each individual single‐guide ribonucleic acid (sgRNA) targeting sequence, two complementary annealed oligonucleotides with appropriate sticky end overhangs were ligated (NEB; #M0202M) into the desired Article Title: Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria Article Snippet: For cloning, CRISPRi plasmids were digested with BsmBI-v2 (NEB #R0739L) and further gel purified (Macherey-Nagel; #740609.50). .. For each individual single-guide ribonucleic acid (sgRNA) targeting sequence, two complementary annealed oligonucleotides with appropriate sticky end overhangs were ligated (NEB; #M0202M) into the desired Sequencing:Article Title: Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria. Article Snippet: For cloning, CRISPRi plasmids were digested with BsmBI-v2 (NEB #R0739L) and further gel purified (Macherey-Nagel, Hoerdt, France; #740609.50). .. For each individual single-guide ribonucleic acid (sgRNA) targeting sequence, two complementary annealed oligonucleotides with appropriate sticky end overhangs were ligated (NEB; #M0202M) into the desired Article Title: Fluorescence‐based CRISPR interference system for controlled genetic repression and live single‐cell imaging in mycobacteria Article Snippet: For cloning, CRISPRi plasmids were digested with BsmBI‐v2 (NEB #R0739L) and further gel purified (Macherey‐Nagel, Hoerdt, France; #740609.50). .. For each individual single‐guide ribonucleic acid (sgRNA) targeting sequence, two complementary annealed oligonucleotides with appropriate sticky end overhangs were ligated (NEB; #M0202M) into the desired Article Title: Fluorescence-based CRISPR interference system for controlled genetic repression and live single-cell imaging in mycobacteria Article Snippet: For cloning, CRISPRi plasmids were digested with BsmBI-v2 (NEB #R0739L) and further gel purified (Macherey-Nagel; #740609.50). .. For each individual single-guide ribonucleic acid (sgRNA) targeting sequence, two complementary annealed oligonucleotides with appropriate sticky end overhangs were ligated (NEB; #M0202M) into the desired |